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Microbiology Chapter 5 Test Answers

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    Q3 How is pH important in microbial growth -most bacteria 6. Water leaves cell, cell can die. Example: meat packed in salt. Like high salt outside. Q3 Define facultative halophles - can tolerate high salt concentration but not required. Q6 Distinguish between chemically defined media and complex media -both are culture media that are nutrients prepared for microbial growth. CDM - exact chemical composition known. Complex Media: extract and digest of yeasts, meats or plants: nutrient broth liquid or nutrient agar solid. Ex: Blood Agar. Procedures to increase small levels of a microbe to detectable levels. If microbe can only grow using phonol for carbon and energy, put it in that culture. After growth period, put small sample in another plate. Q7 What are anaerobic media and techniques? Media used to grow anaerobes o2 deadly. Called Reducing Media. Contains ingredients combinewith dissolved oxygen and deplete it. They are heated.

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    Q7 What is a candle jars? Candle Jar: used to create a high CO2, low oxygen environment within a jar. Cultures in Jar, sealed, candle lit,goes out whe O2 low. Q8 What is a capnophiles? Microbes that grow better in high CO2, low O2 concentrations, such as a candle jar. Q7 What are living host cells? Used when a bacteria can't be grown in an artificial laboratory media. Example Leprocy bacillus. Last cells rubbed grow into isolated colonies.

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    Can be transferred to test tube to form a pure culture. Q9 Define Generation Time Generation Time: the time required for a cell to divide and its population to double. Varies greatly among bacteria, from 1 to 3 hrs, some greater than 24 hrs. Q10 Name and describe phases of the bacterial growth curve. Q11 What are several direct measurements of microbial growth? Filtration, and Direct Microscopic Count Q11 What are several indirect measurements of microbe growth? Q12 Terms for destruction or supression of microbial growth: -Sterilization.

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